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Sample GSM1521074 Query DataSets for GSM1521074
Status Public on Oct 08, 2014
Title HEK293_siMAML1 (DNA)
Sample type genomic
Source name HEK293 cells, siMAML1 knock-down, rep 1
Organism Homo sapiens
Characteristics cell line: HEK293 human embryonic kidney cells
treatment: siMAML1 knock-down
Treatment protocol The MAML1 cell lines were constructed by transfecting human embryonic kidney HEK293 cells (ATCC, Manassas, VA, USA) with the pcDNA3.1-MAML1 vector, and stable clones were selected with geneticin. For siRNA experiments, HEK293 cells were transiently transfected with 100 nM MAML1 siRNA (a predesigned MAML1 SMARTpool set of 4 siRNAs; Dharmacon, Lafayette Colorado, USA) or control siRNA using DharmaFECT 1 siRNA reagents (Dharmacon) in 6-well plates. DNA was collected 72 hours post-transfection.
Growth protocol HEK293 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (all from Gibco, Life Technologies).
Extracted molecule genomic DNA
Extraction protocol Invisorb Spin Tissue Mini Kit (Invitek, Nordic Biolabs)
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Sample name: HEK293_siMAML
Data processing Illumina GenomeStudio software (v. 2010.2)
Submission date Oct 08, 2014
Last update date Oct 08, 2014
Contact name Milica Putnik
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
Platform ID GPL13534
Series (2)
GSE62177 Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells (methylation)
GSE62178 Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells

Data table header descriptions
VALUE Average Beta

Data table
cg00000029 0.7951093
cg00000108 0.8436545
cg00000109 0.8502901
cg00000165 0.925779
cg00000236 0.8461181
cg00000289 0.6234844
cg00000292 0.8993542
cg00000321 0.8091978
cg00000363 0.8535312
cg00000622 0.02357103
cg00000658 0.7960269
cg00000714 0.3373671
cg00000721 0.9114432
cg00000734 0.08826628
cg00000769 0.06354745
cg00000807 0.8619747
cg00000884 0.764081
cg00000905 0.1621554
cg00000924 0.4915357
cg00000948 0.8956113

Total number of rows: 485577

Table truncated, full table size 10009 Kbytes.

Supplementary file Size Download File type/resource
GSM1521074_6026818158_R02C01_Grn.idat.gz 4.1 Mb (ftp)(http) IDAT
GSM1521074_6026818158_R02C01_Red.idat.gz 4.1 Mb (ftp)(http) IDAT
Processed data included within Sample table
Processed data are available on Series record

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