|
| Status |
Public on Oct 08, 2014 |
| Title |
HEK293_siMAML1 (DNA) |
| Sample type |
genomic |
| |
|
| Source name |
HEK293 cells, siMAML1 knock-down, rep 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293 human embryonic kidney cells
treatment: siMAML1 knock-down
|
| Treatment protocol |
The MAML1 cell lines were constructed by transfecting human embryonic kidney HEK293 cells (ATCC, Manassas, VA, USA) with the pcDNA3.1-MAML1 vector, and stable clones were selected with geneticin. For siRNA experiments, HEK293 cells were transiently transfected with 100 nM MAML1 siRNA (a predesigned MAML1 SMARTpool set of 4 siRNAs; Dharmacon, Lafayette Colorado, USA) or control siRNA using DharmaFECT 1 siRNA reagents (Dharmacon) in 6-well plates. DNA was collected 72 hours post-transfection.
|
| Growth protocol |
HEK293 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (all from Gibco, Life Technologies).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Invisorb Spin Tissue Mini Kit (Invitek, Nordic Biolabs)
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
Sample name: HEK293_siMAML
|
| Data processing |
Illumina GenomeStudio software (v. 2010.2)
|
| |
|
| Submission date |
Oct 08, 2014 |
| Last update date |
Oct 08, 2014 |
| Contact name |
Milica Putnik |
| Organization name |
Karolinska Institute
|
| Department |
Biosciences and Nutrition
|
| Street address |
Hälsovägen 7
|
| City |
Huddinge |
| ZIP/Postal code |
14183 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE62177 |
Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells (methylation) |
| GSE62178 |
Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells |
|
