GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1521073 Query DataSets for GSM1521073
Status Public on Oct 08, 2014
Title HEK293_siControl (DNA)
Sample type genomic
Source name HEK293 cells, siControl knock-down, rep 1
Organism Homo sapiens
Characteristics cell line: HEK293 human embryonic kidney cells
treatment: siControl knock-down
Treatment protocol The MAML1 cell lines were constructed by transfecting human embryonic kidney HEK293 cells (ATCC, Manassas, VA, USA) with the pcDNA3.1-MAML1 vector, and stable clones were selected with geneticin. For siRNA experiments, HEK293 cells were transiently transfected with 100 nM MAML1 siRNA (a predesigned MAML1 SMARTpool set of 4 siRNAs; Dharmacon, Lafayette Colorado, USA) or control siRNA using DharmaFECT 1 siRNA reagents (Dharmacon) in 6-well plates. DNA was collected 72 hours post-transfection.
Growth protocol HEK293 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (all from Gibco, Life Technologies).
Extracted molecule genomic DNA
Extraction protocol Invisorb Spin Tissue Mini Kit (Invitek, Nordic Biolabs)
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Sample name: HEK293_siControl
Data processing Illumina GenomeStudio software (v. 2010.2)
Submission date Oct 08, 2014
Last update date Oct 08, 2014
Contact name Milica Putnik
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
Platform ID GPL13534
Series (2)
GSE62177 Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells (methylation)
GSE62178 Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells

Data table header descriptions
VALUE Average Beta

Data table
cg00000029 0.8308934
cg00000108 0.910347
cg00000109 0.8657925
cg00000165 0.9029667
cg00000236 0.8729526
cg00000289 0.7190184
cg00000292 0.8658861
cg00000363 0.8399668
cg00000622 0.01940859
cg00000658 0.800919
cg00000714 0.3338557
cg00000721 0.9221984
cg00000734 0.09873003
cg00000769 0.05581876
cg00000807 0.8590925
cg00000884 0.7733033
cg00000905 0.1147213
cg00000924 0.4965123
cg00000948 0.8899585

Total number of rows: 485577

Table truncated, full table size 10012 Kbytes.

Supplementary file Size Download File type/resource
GSM1521073_6026818158_R01C01_Grn.idat.gz 4.1 Mb (ftp)(http) IDAT
GSM1521073_6026818158_R01C01_Red.idat.gz 4.1 Mb (ftp)(http) IDAT
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap