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Sample GSM1521071 Query DataSets for GSM1521071
Status Public on Oct 08, 2014
Title HEK293_MAML1_1 (DNA)
Sample type genomic
Source name HEK293 cells, MAML1 stable overexpression, rep 1
Organism Homo sapiens
Characteristics cell line: HEK293 human embryonic kidney cells
treatment: MAML1 stable overexpression
Treatment protocol The MAML1 cell lines were constructed by transfecting human embryonic kidney HEK293 cells (ATCC, Manassas, VA, USA) with the pcDNA3.1-MAML1 vector, and stable clones were selected with geneticin. For siRNA experiments, HEK293 cells were transiently transfected with 100 nM MAML1 siRNA (a predesigned MAML1 SMARTpool set of 4 siRNAs; Dharmacon, Lafayette Colorado, USA) or control siRNA using DharmaFECT 1 siRNA reagents (Dharmacon) in 6-well plates. DNA was collected 72 hours post-transfection.
Growth protocol HEK293 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (all from Gibco, Life Technologies).
Extracted molecule genomic DNA
Extraction protocol Invisorb Spin Tissue Mini Kit (Invitek, Nordic Biolabs)
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Sample name: HEK293_MAML1 1
Data processing Illumina GenomeStudio software (v. 2010.2)
Submission date Oct 08, 2014
Last update date Oct 08, 2014
Contact name Milica Putnik
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
Platform ID GPL13534
Series (2)
GSE62177 Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells (methylation)
GSE62178 Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells

Data table header descriptions
VALUE Average Beta

Data table
cg00000029 0.9151399
cg00000108 0.8947889
cg00000109 0.8831316
cg00000165 0.9417917
cg00000236 0.8353533
cg00000289 0.7635336
cg00000292 0.8661448
cg00000321 0.8222486
cg00000363 0.8931046
cg00000622 0.02059994
cg00000658 0.7925196
cg00000714 0.4645529
cg00000721 0.9172211
cg00000734 0.09621608
cg00000769 0.06300659
cg00000807 0.8883412
cg00000884 0.7619507
cg00000905 0.1041205
cg00000924 0.5198761
cg00000948 0.9119947

Total number of rows: 485577

Table truncated, full table size 10007 Kbytes.

Supplementary file Size Download File type/resource
GSM1521071_5684819031_R03C02_Grn.idat.gz 4.1 Mb (ftp)(http) IDAT
GSM1521071_5684819031_R03C02_Red.idat.gz 4.1 Mb (ftp)(http) IDAT
Processed data included within Sample table
Processed data are available on Series record

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