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Sample GSM1521069 Query DataSets for GSM1521069
Status Public on Oct 08, 2014
Title HEK293_control_1 (DNA)
Sample type genomic
Source name HEK293 cells, no treatment, rep 1
Organism Homo sapiens
Characteristics cell line: HEK293 human embryonic kidney cells
treatment: no treatment
Treatment protocol The MAML1 cell lines were constructed by transfecting human embryonic kidney HEK293 cells (ATCC, Manassas, VA, USA) with the pcDNA3.1-MAML1 vector, and stable clones were selected with geneticin. For siRNA experiments, HEK293 cells were transiently transfected with 100 nM MAML1 siRNA (a predesigned MAML1 SMARTpool set of 4 siRNAs; Dharmacon, Lafayette Colorado, USA) or control siRNA using DharmaFECT 1 siRNA reagents (Dharmacon) in 6-well plates. DNA was collected 72 hours post-transfection.
Growth protocol HEK293 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (all from Gibco, Life Technologies).
Extracted molecule genomic DNA
Extraction protocol Invisorb Spin Tissue Mini Kit (Invitek, Nordic Biolabs)
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Sample name: HEK293 1
Data processing Illumina GenomeStudio software (v. 2010.2)
Submission date Oct 08, 2014
Last update date Oct 08, 2014
Contact name Milica Putnik
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
Platform ID GPL13534
Series (2)
GSE62177 Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells (methylation)
GSE62178 Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells

Data table header descriptions
VALUE Average Beta

Data table
cg00000029 0.8959613
cg00000108 0.9150077
cg00000109 0.8688241
cg00000165 0.8925989
cg00000236 0.8871546
cg00000289 0.7700534
cg00000292 0.8601949
cg00000321 0.7001186
cg00000363 0.8895637
cg00000622 0.0165795
cg00000658 0.7941263
cg00000714 0.4752724
cg00000721 0.903841
cg00000734 0.08539918
cg00000769 0.06532918
cg00000807 0.8620009
cg00000884 0.6606517
cg00000905 0.1898389
cg00000924 0.5259875
cg00000948 0.9119598

Total number of rows: 485577

Table truncated, full table size 9996 Kbytes.

Supplementary file Size Download File type/resource
GSM1521069_5684819031_R01C01_Grn.idat.gz 4.1 Mb (ftp)(http) IDAT
GSM1521069_5684819031_R01C01_Red.idat.gz 4.1 Mb (ftp)(http) IDAT
Processed data included within Sample table
Processed data are available on Series record

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