|Public on Nov 13, 2014
|cell type: MOLT3
chip antibody: MYB
antibody manu.: Abcam
antibody catalog: ab45150
tal1 enhancer status: WT/MOLT3 WT
|Cells were grown according to ATCC guidelines
|Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies.
|Illumina HiSeq 2000
|Images analysis and base calling was done using the solexa pipeline.
Aligned using /usr/local/bin/bowtie with configuration -p 4 --best -k 2 -m 2 --sam -l 40
Supplementary_files_format_and_content: WIG files(s) represent counts of aligned reads within 50 bp bins with each read being extended 200 in the direction of alignment. Counts are in reads-per-million and floored at 0.1
|Oct 06, 2014
|Last update date
|May 15, 2019
|Richard A Young
|Whitehead Institute for Biomedical Research
|9 Cambridge Center
|An Oncogenic Super-Enhancer Formed through Somatic Mutation of a Noncoding Intergenic Element