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Sample GSM1519605 Query DataSets for GSM1519605
Status Public on Oct 07, 2014
Title CD161-_Patient4
Sample type RNA
Source name CD8+ T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD8+ T cells
Treatment protocol CD8+ T cell subsets were sorted using a FACS sorter
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in FACS sorted CD161- CD8+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62096 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD 8)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 6.802095967 0
ILMN_1660305 7.813626058 0
ILMN_1792173 8.514285707 0
ILMN_1762337 6.048403088 0.60876
ILMN_2055271 6.206715285 0.12859
ILMN_1736007 6.247676588 0.07524
ILMN_1814316 6.007509705 0.73187
ILMN_2359168 6.090129044 0.4528
ILMN_1731507 5.87283136 0.97401
ILMN_1787689 6.088169222 0.45554
ILMN_1745607 6.215719376 0.11491
ILMN_2136495 6.149599532 0.25308
ILMN_1668111 6.099888419 0.42818
ILMN_2295559 6.124958078 0.32011
ILMN_1735045 5.961146331 0.8591
ILMN_1680754 6.050417644 0.60602
ILMN_2375184 6.080303201 0.49384
ILMN_1659452 6.221094979 0.10944
ILMN_1767388 6.224667619 0.10397
ILMN_1675204 5.913243381 0.93981

Total number of rows: 48803

Table truncated, full table size 1463 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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