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Sample GSM1519604 Query DataSets for GSM1519604
Status Public on Oct 07, 2014
Title CD161++_Patient3
Sample type RNA
Source name CD8+ T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD8+ T cells
Treatment protocol CD8+ T cell subsets were sorted using a FACS sorter
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in FACS sorted CD161++ CD8+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62096 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD 8)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 6.60651204 0
ILMN_1660305 7.754546821 0
ILMN_1792173 7.922342063 0
ILMN_1762337 6.065490133 0.91216
ILMN_2055271 6.255071769 0.16892
ILMN_1736007 6.220552599 0.31081
ILMN_1814316 6.21763837 0.32432
ILMN_2359168 6.147402511 0.64459
ILMN_1731507 6.108601546 0.79865
ILMN_1787689 6.127356286 0.72838
ILMN_1745607 6.153514958 0.60541
ILMN_2136495 6.327144983 0.05405
ILMN_1668111 6.047550081 0.93919
ILMN_2295559 6.141264056 0.67568
ILMN_1735045 6.164149819 0.55541
ILMN_1680754 6.255071769 0.17297
ILMN_2375184 6.099131948 0.83243
ILMN_1659452 6.256492297 0.16757
ILMN_1767388 6.309461415 0.07432
ILMN_1675204 6.045908051 0.94189

Total number of rows: 48803

Table truncated, full table size 1461 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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