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Sample GSM1519602 Query DataSets for GSM1519602
Status Public on Oct 07, 2014
Title CD161-_Patient3
Sample type RNA
Source name CD8+ T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD8+ T cells
Treatment protocol CD8+ T cell subsets were sorted using a FACS sorter
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in FACS sorted CD161- CD8+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62096 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD 8)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 6.938233625 0
ILMN_1660305 8.06673688 0
ILMN_1792173 8.443392698 0
ILMN_1762337 5.843602673 0.95342
ILMN_2055271 6.150926505 0.15068
ILMN_1736007 6.194773086 0.08082
ILMN_1814316 5.911442534 0.85068
ILMN_2359168 5.967469342 0.71918
ILMN_1731507 5.902272826 0.86986
ILMN_1787689 5.989284714 0.64247
ILMN_1745607 5.895357094 0.89178
ILMN_2136495 6.264417133 0.02466
ILMN_1668111 5.940847855 0.79589
ILMN_2295559 6.050745429 0.43425
ILMN_1735045 6.00650257 0.59452
ILMN_1680754 6.004361542 0.59863
ILMN_2375184 5.940847855 0.79589
ILMN_1659452 6.054889125 0.41644
ILMN_1767388 6.230015315 0.04795
ILMN_1675204 5.98059811 0.68493

Total number of rows: 48803

Table truncated, full table size 1460 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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