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Sample GSM1519601 Query DataSets for GSM1519601
Status Public on Oct 07, 2014
Title CD161++_Patient2
Sample type RNA
Source name CD8+ T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD8+ T cells
Treatment protocol CD8+ T cell subsets were sorted using a FACS sorter
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in FACS sorted CD161++ CD8+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62096 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD 8)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 6.310118906 0.0195
ILMN_1660305 8.231088711 0
ILMN_1792173 8.239836619 0
ILMN_1762337 5.949629808 0.56267
ILMN_2055271 6.255951429 0.04457
ILMN_1736007 6.043220749 0.32033
ILMN_1814316 5.991670134 0.46936
ILMN_2359168 6.029832376 0.36212
ILMN_1731507 5.879708287 0.76045
ILMN_1787689 6.206826924 0.07521
ILMN_1745607 5.809362304 0.88162
ILMN_2136495 6.0378803 0.33565
ILMN_1668111 5.815617354 0.87326
ILMN_2295559 6.230428263 0.05989
ILMN_1735045 5.796770252 0.90669
ILMN_1680754 5.821845402 0.86908
ILMN_2375184 6.027139727 0.3649
ILMN_1659452 6.30790207 0.0195
ILMN_1767388 6.206826924 0.07521
ILMN_1675204 5.821845402 0.86908

Total number of rows: 48803

Table truncated, full table size 1465 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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