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Sample GSM1519600 Query DataSets for GSM1519600
Status Public on Oct 07, 2014
Title CD161+_Patient2
Sample type RNA
Source name CD8+ T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD8+ T cells
Treatment protocol CD8+ T cell subsets were sorted using a FACS sorter
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in FACS sorted CD161+ CD8+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62096 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD 8)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 6.335029109 0
ILMN_1660305 8.447949538 0
ILMN_1792173 8.229980114 0
ILMN_1762337 5.955635891 0.61088
ILMN_2055271 6.18685452 0.05858
ILMN_1736007 6.050573053 0.30544
ILMN_1814316 5.935875071 0.70153
ILMN_2359168 5.946886703 0.65969
ILMN_1731507 5.975129698 0.53696
ILMN_1787689 6.038269959 0.33891
ILMN_1745607 5.861010983 0.90377
ILMN_2136495 6.162499312 0.08926
ILMN_1668111 6.007045221 0.42678
ILMN_2295559 6.137725876 0.12134
ILMN_1735045 6.078878712 0.21757
ILMN_1680754 5.877219104 0.86192
ILMN_2375184 6.080879452 0.21618
ILMN_1659452 6.042382659 0.32357
ILMN_1767388 6.14730453 0.10321
ILMN_1675204 6.013344467 0.40167

Total number of rows: 48803

Table truncated, full table size 1460 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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