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Sample GSM1519599 Query DataSets for GSM1519599
Status Public on Oct 07, 2014
Title CD161-_Patient2
Sample type RNA
Source name CD8+ T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD8+ T cells
Treatment protocol CD8+ T cell subsets were sorted using a FACS sorter
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in FACS sorted CD161- CD8+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62096 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD 8)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 6.70016051 0
ILMN_1660305 8.067672158 0
ILMN_1792173 8.278045588 0
ILMN_1762337 5.939074844 0.66205
ILMN_2055271 6.407684465 0
ILMN_1736007 6.124686307 0.14681
ILMN_1814316 5.919855299 0.72299
ILMN_2359168 5.769603362 0.97368
ILMN_1731507 6.031509824 0.36704
ILMN_1787689 6.042658638 0.32825
ILMN_1745607 5.890537205 0.81579
ILMN_2136495 6.126783185 0.13989
ILMN_1668111 6.071248672 0.25762
ILMN_2295559 5.905270725 0.78255
ILMN_1735045 5.895465115 0.80194
ILMN_1680754 5.912581442 0.75346
ILMN_2375184 6.029269685 0.37673
ILMN_1659452 5.8681509 0.8615
ILMN_1767388 6.118377323 0.15374
ILMN_1675204 5.669592262 1

Total number of rows: 48803

Table truncated, full table size 1460 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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