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Sample GSM1519597 Query DataSets for GSM1519597
Status Public on Oct 07, 2014
Title CD161+_Patient1
Sample type RNA
Source name CD8+ T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD8+ T cells
Treatment protocol CD8+ T cell subsets were sorted using a FACS sorter
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in FACS sorted CD161+ CD8+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62096 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD 8)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 6.56516566 0
ILMN_1660305 8.128977206 0
ILMN_1792173 7.935874464 0
ILMN_1762337 6.006689293 0.75172
ILMN_2055271 6.235804694 0.07586
ILMN_1736007 6.244728381 0.06897
ILMN_1814316 5.914631598 0.9531
ILMN_2359168 6.098155675 0.41655
ILMN_1731507 5.987324555 0.80828
ILMN_1787689 5.992631677 0.78897
ILMN_1745607 6.011925785 0.73655
ILMN_2136495 6.151324861 0.22621
ILMN_1668111 5.974864815 0.8469
ILMN_2295559 6.089926022 0.44552
ILMN_1735045 6.081649155 0.48
ILMN_1680754 6.305694877 0.02483
ILMN_2375184 6.064951589 0.54069
ILMN_1659452 6.119335626 0.33379
ILMN_1767388 6.119335626 0.33517
ILMN_1675204 6.020611253 0.70069

Total number of rows: 48803

Table truncated, full table size 1460 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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