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Sample GSM1519596 Query DataSets for GSM1519596
Status Public on Oct 07, 2014
Title CD161-_Patient1
Sample type RNA
Source name CD8+ T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD8+ T cells
Treatment protocol CD8+ T cell subsets were sorted using a FACS sorter
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in FACS sorted CD161- CD8+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62096 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD 8)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1809034 6.934404588 0
ILMN_1660305 7.934792451 0
ILMN_1792173 8.014272425 0
ILMN_1762337 6.056668591 0.4903
ILMN_2055271 6.245669087 0.04432
ILMN_1736007 6.175514933 0.1205
ILMN_1814316 5.961992591 0.84765
ILMN_2359168 5.92900102 0.90859
ILMN_1731507 6.147107049 0.1759
ILMN_1787689 6.063440548 0.45014
ILMN_1745607 6.105059961 0.29224
ILMN_2136495 6.201840522 0.07895
ILMN_1668111 5.990698177 0.76177
ILMN_2295559 6.049864696 0.51108
ILMN_1735045 6.109974559 0.27701
ILMN_1680754 6.081901176 0.35873
ILMN_2375184 6.096831577 0.30748
ILMN_1659452 6.145512311 0.18144
ILMN_1767388 6.232209059 0.05125
ILMN_1675204 5.947422732 0.87396

Total number of rows: 48803

Table truncated, full table size 1454 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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