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Sample GSM1519595 Query DataSets for GSM1519595
Status Public on Oct 07, 2014
Title CD161+_3
Sample type RNA
 
Source name CD4 T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD4+ T cells
Treatment protocol CD4+ T cells were enriched using EasySep human CD4 enrichment kit, and CD161+ and CD161- cells then selected by anti-PE microbeads (Miltenyi Biotec)
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
 
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in CD161+ CD4+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
 
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
 
Platform ID GPL6884
Series (2)
GSE62095 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD4)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
ID_REF
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.934945338 0.37681
ILMN_2055271 6.362171643 0.05797
ILMN_1736007 5.990061888 0.31357
ILMN_2383229 5.713756037 0.60606
ILMN_1806310 5.65357525 0.65086
ILMN_1779670 6.289332866 0.08696
ILMN_2321282 5.726795349 0.58762
ILMN_1671474 6.182176027 0.1502
ILMN_1772582 5.513875129 0.77075
ILMN_1735698 6.524049542 0.01976
ILMN_1653355 6.748422617 0.00527
ILMN_1717783 5.677278094 0.62978
ILMN_1705025 6.262721098 0.09881
ILMN_1814316 5.636403006 0.66535
ILMN_2359168 5.833114524 0.49275
ILMN_1731507 5.683979419 0.62582
ILMN_1787689 5.113272492 0.90909
ILMN_1745607 5.865899664 0.4585
ILMN_2136495 5.889283977 0.42688
ILMN_1668111 5.673915723 0.63373

Total number of rows: 48802

Table truncated, full table size 1502 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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