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Sample GSM1519594 Query DataSets for GSM1519594
Status Public on Oct 07, 2014
Title CD161-_3
Sample type RNA
Source name CD4 T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD4+ T cells
Treatment protocol CD4+ T cells were enriched using EasySep human CD4 enrichment kit, and CD161+ and CD161- cells then selected by anti-PE microbeads (Miltenyi Biotec)
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in CD161- CD4+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62095 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD4)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.660005053 0.62055
ILMN_2055271 6.308998389 0.05007
ILMN_1736007 6.063793376 0.18182
ILMN_2383229 6.012949884 0.24506
ILMN_1806310 5.343489297 0.86034
ILMN_1779670 6.08363945 0.16469
ILMN_2321282 6.002562575 0.25428
ILMN_1671474 6.143955218 0.11858
ILMN_1772582 5.626574047 0.65481
ILMN_1735698 6.582684281 0.01186
ILMN_1653355 6.914493447 0.00264
ILMN_1717783 5.442870347 0.79842
ILMN_1705025 6.204118361 0.09091
ILMN_1814316 5.581922372 0.69565
ILMN_2359168 5.970944239 0.27273
ILMN_1731507 5.524998806 0.74308
ILMN_1787689 5.708742437 0.57444
ILMN_1745607 5.686203053 0.59684
ILMN_2136495 6.139221675 0.12121
ILMN_1668111 5.848694299 0.42292

Total number of rows: 48802

Table truncated, full table size 1505 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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