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Sample GSM1519591 Query DataSets for GSM1519591
Status Public on Oct 07, 2014
Title CD161+_1
Sample type RNA
Source name CD4 T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD4+ T cells
Treatment protocol CD4+ T cells were enriched using EasySep human CD4 enrichment kit, and CD161+ and CD161- cells then selected by anti-PE microbeads (Miltenyi Biotec)
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in CD161+ CD4+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62095 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD4)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 6.017408421 0.52701
ILMN_2055271 6.513789734 0.11067
ILMN_1736007 6.151882501 0.41634
ILMN_2383229 6.086211688 0.47167
ILMN_1806310 5.972341609 0.56917
ILMN_1779670 6.513789734 0.10935
ILMN_2321282 5.991069332 0.54941
ILMN_1671474 6.537643935 0.09223
ILMN_1772582 5.903399545 0.6166
ILMN_1735698 6.715482948 0.02767
ILMN_1653355 6.575363409 0.07246
ILMN_1717783 6.017408421 0.52569
ILMN_1705025 6.802874433 0.00922
ILMN_1814316 6.066165385 0.49144
ILMN_2359168 6.014796047 0.52964
ILMN_1731507 6.078726928 0.47694
ILMN_1787689 5.214135067 0.8946
ILMN_1745607 5.553488313 0.8195
ILMN_2136495 6.500779905 0.11462
ILMN_1668111 5.928599882 0.60606

Total number of rows: 48802

Table truncated, full table size 1509 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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