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Sample GSM1519590 Query DataSets for GSM1519590
Status Public on Oct 07, 2014
Title CD161-_1
Sample type RNA
Source name CD4 T cells
Organism Homo sapiens
Characteristics tissue: Peripheral Blood cells
cell type: CD4+ T cells
Treatment protocol CD4+ T cells were enriched using EasySep human CD4 enrichment kit, and CD161+ and CD161- cells then selected by anti-PE microbeads (Miltenyi Biotec)
Growth protocol Cells taken ex vivo from healthy donors and separated using Ficoll centrifugation
Extracted molecule total RNA
Extraction protocol RNA was extracted from cells using a Qiagen Rneasy kit
Label Cy3
Label protocol Biotinylated cRNA Probes were prepared using illumina TotalPrep-96 Ambion # 4393543
Hybridization protocol A total of 750ng of biotin-labeled cRNA from each sample was used in the hybridization process at 58C for 18 h. The hybridized BeadChip was washed and labeled with streptavidin-Cy3 according to the Illumina protocols.
Scan protocol Chips were scanned with Illumina BeadArray Reader accordingly
Description Gene expression in CD161- CD4+ T cells
Data processing GenomeStudio (Illumina Inc) was used for data extraction accordingly
Submission date Oct 06, 2014
Last update date Oct 07, 2014
Contact name Joannah R Fergusson
Organization name University of Oxford
Department Peter Medawar Building for Pathogen Research
Street address South Parks Road
City Oxford
State/province select a state
ZIP/Postal code OX1 3SY
Country United Kingdom
Platform ID GPL6884
Series (2)
GSE62095 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages (CD4)
GSE62099 CD161 defines a transcriptional and functional phenotype shared across distinct human T cell lineages

Data table header descriptions
VALUE vsn-normalised signal intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.938806467 0.43874
ILMN_2055271 6.114899477 0.23847
ILMN_1736007 5.779699952 0.62714
ILMN_2383229 6.200594024 0.16074
ILMN_1806310 5.700266369 0.69829
ILMN_1779670 6.546208824 0.01581
ILMN_2321282 5.661316561 0.72991
ILMN_1671474 6.368692948 0.05402
ILMN_1772582 5.914168047 0.4664
ILMN_1735698 6.571376455 0.01449
ILMN_1653355 6.423550767 0.04084
ILMN_1717783 5.707453822 0.68906
ILMN_1705025 6.322629555 0.07246
ILMN_1814316 5.813400207 0.59157
ILMN_2359168 6.021870941 0.3531
ILMN_1731507 5.636427887 0.7444
ILMN_1787689 5.3205585 0.88011
ILMN_1745607 5.817834677 0.58103
ILMN_2136495 6.34123177 0.06719
ILMN_1668111 5.880648298 0.50725

Total number of rows: 48802

Table truncated, full table size 1505 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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