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Sample GSM151916 Query DataSets for GSM151916
Status Public on Mar 05, 2007
Title HS578T (U133B)
Sample type RNA
 
Source name cultured human breast cancer cell
Organism Homo sapiens
Characteristics Dasatinib sensitive
Extracted molecule total RNA
Extraction protocol Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cultured breast cancer cell line
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Dec 19, 2006
Last update date Jul 20, 2007
Contact name Fei Huang
Organization name Bristol-Myers Squibb Co
Street address P.O. Box 5400/HW3B2.02
City Princeton
State/province NJ
ZIP/Postal code 08543
Country USA
 
Platform ID GPL97
Series (1)
GSE6569 Expression data from 23 breast cancer cell lines

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
200000_s_at 30544 P
200001_at 61324.8 P
200002_at 116614.9 P
200003_s_at 188680.6 P
200004_at 87332.3 P
200005_at 45216.1 P
200006_at 109348.1 P
200007_at 49040.9 P
200008_s_at 18342.3 P
200009_at 39323 P
200010_at 117707.3 P
200011_s_at 14476.6 P
200012_x_at 100428.8 P
200013_at 182066 P
200014_s_at 23822.6 P
200015_s_at 34697.9 P
200016_x_at 101287.9 P
200017_at 127081.8 P
200018_at 142472.3 P
200019_s_at 102425.3 P

Total number of rows: 22645

Table truncated, full table size 409 Kbytes.




Supplementary data files not provided

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