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Sample GSM151914 Query DataSets for GSM151914
Status Public on Mar 05, 2007
Title BT-20 (U133B)
Sample type RNA
 
Source name cultured human breast cancer cell
Organism Homo sapiens
Characteristics Dasatinib sensitive
Extracted molecule total RNA
Extraction protocol Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cultured breast cancer cell line
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Dec 19, 2006
Last update date Jul 20, 2007
Contact name Fei Huang
Organization name Bristol-Myers Squibb Co
Street address P.O. Box 5400/HW3B2.02
City Princeton
State/province NJ
ZIP/Postal code 08543
Country USA
 
Platform ID GPL97
Series (1)
GSE6569 Expression data from 23 breast cancer cell lines

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
200000_s_at 19145.8 P
200001_at 48407 P
200002_at 86405.2 P
200003_s_at 100556 P
200004_at 55864.9 P
200005_at 27568.2 P
200006_at 68422 P
200007_at 59421.8 P
200008_s_at 12332.4 P
200009_at 23788.9 P
200010_at 67919.9 P
200011_s_at 22072.1 P
200012_x_at 87487.5 P
200013_at 130869.9 P
200014_s_at 16680.2 P
200015_s_at 29949 P
200016_x_at 55227.1 P
200017_at 89037.8 P
200018_at 106114.9 P
200019_s_at 67718.8 P

Total number of rows: 22645

Table truncated, full table size 409 Kbytes.




Supplementary data files not provided

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