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Sample GSM151905 Query DataSets for GSM151905
Status Public on Mar 05, 2007
Title MCF7 (U133A)
Sample type RNA
 
Source name cultured human breast cancer cell
Organism Homo sapiens
Characteristics Dasatinib resistant
Extracted molecule total RNA
Extraction protocol Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cultured breast cancer cell line
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Dec 19, 2006
Last update date Jul 20, 2007
Contact name Fei Huang
Organization name Bristol-Myers Squibb Co
Street address P.O. Box 5400/HW3B2.02
City Princeton
State/province NJ
ZIP/Postal code 08543
Country USA
 
Platform ID GPL96
Series (1)
GSE6569 Expression data from 23 breast cancer cell lines

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1007_s_at 7385.7 P
1053_at 1821 P
117_at 442.7 A
121_at 2982.6 P
1255_g_at 118.7 A
1294_at 692.6 P
1316_at 353.9 P
1320_at 59.9 A
1405_i_at 16.8 A
1431_at 293.5 P
1438_at 1611.8 P
1487_at 1738.1 P
1494_f_at 547.4 A
1598_g_at 1695.2 A
160020_at 1358.8 P
1729_at 1585.3 P
177_at 214.2 A
1773_at 764.9 P
179_at 2056.5 P
1861_at 822.6 P

Total number of rows: 22283

Table truncated, full table size 412 Kbytes.




Supplementary data files not provided

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