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Sample GSM151903 Query DataSets for GSM151903
Status Public on Mar 05, 2007
Title BT-549 (U133A)
Sample type RNA
 
Source name cultured human breast cancer cell
Organism Homo sapiens
Characteristics Dasatinib resistant
Extracted molecule total RNA
Extraction protocol Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cultured breast cancer cell line
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Dec 19, 2006
Last update date Jul 20, 2007
Contact name Fei Huang
Organization name Bristol-Myers Squibb Co
Street address P.O. Box 5400/HW3B2.02
City Princeton
State/province NJ
ZIP/Postal code 08543
Country USA
 
Platform ID GPL96
Series (1)
GSE6569 Expression data from 23 breast cancer cell lines

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1007_s_at 1799.7 P
1053_at 2329.8 P
117_at 400.7 P
121_at 4109 P
1255_g_at 132.6 A
1294_at 986.5 A
1316_at 396.6 P
1320_at 466.1 A
1405_i_at 712.2 P
1431_at 156.4 M
1438_at 83.5 A
1487_at 1865.3 P
1494_f_at 860.2 P
1598_g_at 6591.8 P
160020_at 3756.6 P
1729_at 1135.8 P
177_at 625.7 P
1773_at 611.5 A
179_at 3026.8 P
1861_at 1039.3 P

Total number of rows: 22283

Table truncated, full table size 412 Kbytes.




Supplementary data files not provided

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