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Sample GSM151902 Query DataSets for GSM151902
Status Public on Mar 05, 2007
Title H3396 (U133A)
Sample type RNA
 
Source name cultured human breast cancer cell
Organism Homo sapiens
Characteristics Dasatinib resistant
Extracted molecule total RNA
Extraction protocol Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cultured breast cancer cell line
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Dec 19, 2006
Last update date Jul 20, 2007
Contact name Fei Huang
Organization name Bristol-Myers Squibb Co
Street address P.O. Box 5400/HW3B2.02
City Princeton
State/province NJ
ZIP/Postal code 08543
Country USA
 
Platform ID GPL96
Series (1)
GSE6569 Expression data from 23 breast cancer cell lines

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1007_s_at 10783.6 P
1053_at 2846.9 P
117_at 280.4 A
121_at 4344.3 P
1255_g_at 276.8 A
1294_at 685.4 A
1316_at 535.9 P
1320_at 104 A
1405_i_at 53 A
1431_at 237 A
1438_at 638.3 A
1487_at 2444.3 P
1494_f_at 1276 P
1598_g_at 1889.4 A
160020_at 1429.5 P
1729_at 760.2 A
177_at 271 A
1773_at 790.1 A
179_at 3220.2 P
1861_at 962.7 P

Total number of rows: 22283

Table truncated, full table size 412 Kbytes.




Supplementary data files not provided

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