|
| Status |
Public on Mar 01, 2015 |
| Title |
PC3 cells treated with SAM, biological replicate 3 |
| Sample type |
genomic |
| |
|
| Source name |
PC3 cells treated with SAM
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC3
cell type: human invasive prostate cancer cell line
treatment: S-adenosylmethionine (SAM)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from human prostate cancer cell lines using the Qiagen DNeasy Kit according to standard instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol.
|
| |
|
| Hybridization protocol |
Bisulfite-converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 BeadChip using standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting.
|
| Data processing |
BeadStudio software v3.2
|
| |
|
| Submission date |
Oct 03, 2014 |
| Last update date |
Mar 01, 2015 |
| Contact name |
Barbara Stefanska |
| E-mail(s) |
barbara.stefanska@ubc.ca
|
| Organization name |
The University of British Columbia
|
| Department |
Land and Food Systems
|
| Lab |
Stefanska Lab
|
| Street address |
2205 East Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE62053 |
S-adenosylmethionine (SAM) changes DNA methylation patterns in prostate cancer cells |
|
