|
| Status |
Public on Oct 04, 2014 |
| Title |
SCLC_tissue_cluster 2 [GTB.32330] |
| Sample type |
RNA |
| |
|
| Source name |
Ganken Tissue Bank
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: frozen tissue
cimp: cluster 2
gender: male
age: 73y
|
| Treatment protocol |
All specimens were grossly dissected and snap-frozen in liquid nitrogen typically within 15 min of removal, and were kept frozen at -80℃ until use
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Fluorescent Labeling of samples according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Hybridization and Microarray Wash according to the manufacturer’s instructions.
|
| Scan protocol |
Agilent SureScan microarray scanner was used for microarray scanning according to the manufacturer’s instructions.
|
| Description |
RNAeasy Mini Kit (Qiagen, Hilden, Germany)
|
| Data processing |
Agilent Feature Extraction software was used to output the Feature Extraction QC report. RNA Integrity Number (RIN) index was calculated for each sample using Agilent 2100 Expert software. In order to reduce experimental biases in microarray analysis due to poor RNA quality, only RNA samples with RIN number > 4 were further processed.
|
| |
|
| Submission date |
Oct 03, 2014 |
| Last update date |
Oct 04, 2014 |
| Contact name |
YUICHI SAITO |
| E-mail(s) |
k3699004@gmail.com
|
| Phone |
81335200111
|
| Organization name |
The Cancer Institute of Japanese Foundation Cancer Research
|
| Department |
Pathology
|
| Street address |
3-8-31 Ariake
|
| City |
Koto |
| State/province |
Tokyo |
| ZIP/Postal code |
1358550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE62021 |
Prediction of prognosis for small cell lung cancer based on genome-wide methylation analyses with surgical materials and robust clustering methods |
|
