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Sample GSM1517178 Query DataSets for GSM1517178
Status Public on Jan 15, 2015
Title CB4_mRNA
Sample type RNA
 
Source name CD19+/CD10+/CD44low/CXCR4+ B cells
Organism Homo sapiens
Characteristics tissue: Tonsil
tumor: none
previously treated for nhl: NA
cell type: centroblasts
Treatment protocol none
Growth protocol none
Extracted molecule total RNA
Extraction protocol RNA was purified using Qiagen RNeasy Kits according to the manufacturer’s protocol.
Label biotin
Label protocol Purified RNA was amplified using Nugen Ovation Pico SL or Ovation Pico kits and labeled using Nugen Encore Biotin kit
 
Hybridization protocol Products were hybridized to Affymetrix Human Gene 1.0 ST arrays according to the manufacturer’s protocol
Scan protocol GS3000
Description Gene expression analysis was performed for centroblasts using Affymetrix Human Gene 2.0 ST arrays
Data processing Gene expression levels were determined using the Affymetrix Expression Console software (v1.2.0.20) with exon-level RMA analysis and default settings
 
Submission date Sep 30, 2014
Last update date Jan 15, 2015
Contact name Jacqueline Payton
E-mail(s) jpayton@wustl.edu
Organization name Washington University School of Medicine
Department Pathology and Immunology
Street address 660 S. Euclid Ave
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL19251
Series (2)
GSE61912 Enhancer Sequence Variants and Transcription Factor Deregulation Synergize to Construct Pathogenic Regulatory Circuits in B Cell Lymphoma (array)
GSE62246 Enhancer Sequence Variants and Transcription Factor Deregulation Synergize to Construct Pathogenic Regulatory Circuits in B Cell Lymphoma

Supplementary file Size Download File type/resource
GSM1517178_CB4.CEL.gz 7.5 Mb (ftp)(http) CEL
GSM1517178_CB4.rma-exon-all-dabg.chp.gz 3.1 Mb (ftp)(http) CHP
Processed data provided as supplementary file

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