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Sample GSM1516172 Query DataSets for GSM1516172
Status Public on Dec 17, 2015
Title MDM-2h-dex100nM-rep4
Sample type RNA
Source name monocyte derived macrophage
Organism Homo sapiens
Characteristics source: fully anonymized volunteers
cell type: monocyte derived macrophage
time: 2h
treatment: dex 100nM
Treatment protocol Samples were treated with 100nM dexamethasone (Sigma) for the indicated duration
Growth protocol CD14+ monocytes were extracted from whole blood donated by healthy volunteers using MACS separation (Miltenyi). They were differentiated in culture for 1 week in the presence of recombinant human CSF1 (10000U/ml),penicillin/streptomycin, Glutamax and 10% fetal calf serum then replated in fresh medium, also supplemented as above, for the time series experiment.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the RNeasy kit (Qiagen) and quality assessed using an 2100 Bioanalyzer (Agilent )
Label biotin
Label protocol Standard affymetrix protocols, Performed by Edinburgh Genomics.
Hybridization protocol Standard affymetrix protocols using GeneTitan. Performed by Edinburgh Genomics.
Scan protocol Standard affymetrix protocols using GeneTitan. Performed by Edinburgh Genomics.
Data processing Raw data was processed using R/Bioconductor: arrayQualityMetrics for quality control and the rma algorithm in the affy package to generate expression values.
Submission date Sep 29, 2014
Last update date Dec 17, 2015
Contact name Alasdair W Jubb
Organization name University of Edinburgh
Department Roslin Institute
Lab Hume lab / Bickmore lab (MRC HGU)
Street address The Roslin Institute
City Easter Bush
State/province Midlothian
ZIP/Postal code EH25 9RG
Country United Kingdom
Platform ID GPL13158
Series (2)
GSE61880 Expression response of human monocyte derived macrophages to dexamethasone over a 24h time series
GSE61881 Divergent transcriptional activation by glucocorticoids in mouse and human macrophages is the result of gain and loss of enhancers

Data table header descriptions
VALUE unlogged rma signal

Data table
1007_PM_s_at 93.43718367
1053_PM_at 156.4870322
117_PM_at 42.44798146
121_PM_at 76.74919653
1255_PM_g_at 6.672470619
1294_PM_at 133.3803224
1316_PM_at 32.29058806
1320_PM_at 6.269356143
1405_PM_i_at 14.02001797
1431_PM_at 7.966499445
1438_PM_at 5.664450379
1487_PM_at 90.7470448
1494_PM_f_at 9.88174901
1552256_PM_a_at 344.0857672
1552257_PM_a_at 174.7093373
1552258_PM_at 7.341765762
1552261_PM_at 5.37881606
1552263_PM_at 291.738293
1552264_PM_a_at 208.6705377
1552266_PM_at 6.490315016

Total number of rows: 54715

Table truncated, full table size 1373 Kbytes.

Supplementary file Size Download File type/resource
GSM1516172_H4_2.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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