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Sample GSM1516159 Query DataSets for GSM1516159
Status Public on Dec 17, 2015
Title MDM-1h-dex100nM-rep2
Sample type RNA
 
Source name monocyte derived macrophage
Organism Homo sapiens
Characteristics source: fully anonymized volunteers
cell type: monocyte derived macrophage
time: 1h
treatment: dex 100nM
Treatment protocol Samples were treated with 100nM dexamethasone (Sigma) for the indicated duration
Growth protocol CD14+ monocytes were extracted from whole blood donated by healthy volunteers using MACS separation (Miltenyi). They were differentiated in culture for 1 week in the presence of recombinant human CSF1 (10000U/ml),penicillin/streptomycin, Glutamax and 10% fetal calf serum then replated in fresh medium, also supplemented as above, for the time series experiment.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the RNeasy kit (Qiagen) and quality assessed using an 2100 Bioanalyzer (Agilent )
Label biotin
Label protocol Standard affymetrix protocols, Performed by Edinburgh Genomics.
 
Hybridization protocol Standard affymetrix protocols using GeneTitan. Performed by Edinburgh Genomics.
Scan protocol Standard affymetrix protocols using GeneTitan. Performed by Edinburgh Genomics.
Data processing Raw data was processed using R/Bioconductor: arrayQualityMetrics for quality control and the rma algorithm in the affy package to generate expression values.
 
Submission date Sep 29, 2014
Last update date Dec 17, 2015
Contact name Alasdair W Jubb
E-mail(s) alasdair.jubb@ed.ac.uk
Organization name University of Edinburgh
Department Roslin Institute
Lab Hume lab / Bickmore lab (MRC HGU)
Street address The Roslin Institute
City Easter Bush
State/province Midlothian
ZIP/Postal code EH25 9RG
Country United Kingdom
 
Platform ID GPL13158
Series (2)
GSE61880 Expression response of human monocyte derived macrophages to dexamethasone over a 24h time series
GSE61881 Divergent transcriptional activation by glucocorticoids in mouse and human macrophages is the result of gain and loss of enhancers

Data table header descriptions
ID_REF
VALUE unlogged rma signal

Data table
ID_REF VALUE
1007_PM_s_at 51.27996894
1053_PM_at 132.0464704
117_PM_at 46.44503861
121_PM_at 85.08151403
1255_PM_g_at 5.223098583
1294_PM_at 108.3270843
1316_PM_at 34.06616557
1320_PM_at 7.378713489
1405_PM_i_at 28.32384267
1431_PM_at 5.159802627
1438_PM_at 6.211254253
1487_PM_at 68.75448964
1494_PM_f_at 9.779324613
1552256_PM_a_at 295.7036702
1552257_PM_a_at 142.9694833
1552258_PM_at 6.947180166
1552261_PM_at 7.7713549
1552263_PM_at 237.871185
1552264_PM_a_at 209.1743843
1552266_PM_at 6.854115012

Total number of rows: 54715

Table truncated, full table size 1373 Kbytes.




Supplementary file Size Download File type/resource
GSM1516159_H2_1.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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