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Sample GSM1516154 Query DataSets for GSM1516154
Status Public on Dec 17, 2015
Title MDM-2h-dex100nM-rep1
Sample type RNA
Source name monocyte derived macrophage
Organism Homo sapiens
Characteristics source: fully anonymized volunteers
cell type: monocyte derived macrophage
time: 2h
treatment: dex 100nM
Treatment protocol Samples were treated with 100nM dexamethasone (Sigma) for the indicated duration
Growth protocol CD14+ monocytes were extracted from whole blood donated by healthy volunteers using MACS separation (Miltenyi). They were differentiated in culture for 1 week in the presence of recombinant human CSF1 (10000U/ml),penicillin/streptomycin, Glutamax and 10% fetal calf serum then replated in fresh medium, also supplemented as above, for the time series experiment.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the RNeasy kit (Qiagen) and quality assessed using an 2100 Bioanalyzer (Agilent )
Label biotin
Label protocol Standard affymetrix protocols, Performed by Edinburgh Genomics.
Hybridization protocol Standard affymetrix protocols using GeneTitan. Performed by Edinburgh Genomics.
Scan protocol Standard affymetrix protocols using GeneTitan. Performed by Edinburgh Genomics.
Data processing Raw data was processed using R/Bioconductor: arrayQualityMetrics for quality control and the rma algorithm in the affy package to generate expression values.
Submission date Sep 29, 2014
Last update date Dec 17, 2015
Contact name Alasdair W Jubb
Organization name University of Edinburgh
Department Roslin Institute
Lab Hume lab / Bickmore lab (MRC HGU)
Street address The Roslin Institute
City Easter Bush
State/province Midlothian
ZIP/Postal code EH25 9RG
Country United Kingdom
Platform ID GPL13158
Series (2)
GSE61880 Expression response of human monocyte derived macrophages to dexamethasone over a 24h time series
GSE61881 Divergent transcriptional activation by glucocorticoids in mouse and human macrophages is the result of gain and loss of enhancers

Data table header descriptions
VALUE unlogged rma signal

Data table
1007_PM_s_at 48.90843163
1053_PM_at 151.1745325
117_PM_at 91.86660512
121_PM_at 45.86496194
1255_PM_g_at 5.498509117
1294_PM_at 133.5188842
1316_PM_at 27.65238061
1320_PM_at 7.180775769
1405_PM_i_at 21.16331348
1431_PM_at 6.080670164
1438_PM_at 6.227032805
1487_PM_at 76.74479147
1494_PM_f_at 12.03054006
1552256_PM_a_at 310.4478309
1552257_PM_a_at 169.8323507
1552258_PM_at 6.986773485
1552261_PM_at 7.392446
1552263_PM_at 308.6680083
1552264_PM_a_at 220.9553932
1552266_PM_at 6.382856137

Total number of rows: 54715

Table truncated, full table size 1373 Kbytes.

Supplementary file Size Download File type/resource
GSM1516154_H1_2.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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