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Status |
Public on Aug 14, 2015 |
Title |
mammillary body, KO, biological rep12 |
Sample type |
RNA |
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Source name |
mammillary neuroepithelium of E10.5 mouse embryos
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Organism |
Mus musculus |
Characteristics |
tissue: brain (mammillary neuroepithelium) strain: C57BL/6 age: E10.5 genotype: Lhx5 KO
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Treatment protocol |
The mammillary neuroepithelium of E10.5 wild type and Lhx5 mutant embryos was dissected and immediately frozen at -80°C. The RNA was preserved with RNAlater ICE (Life Technologies).
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Growth protocol |
Mice were crossed to obtain E10.5 wild type and Lhx5 mutant embryos.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted by using the RNeasy Micro Kit (Qiagen) following the manufacturers instructions for animal tissue.
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Label |
biotin
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Label protocol |
RNA was labeled using the Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit
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Hybridization protocol |
Hybridization was performed for 16 hr at 45°C on GeneChip Mouse Gene 1.1 ST arrays. GeneChips were washed and stained in the Affymetrix Fuidics Station 450.
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Scan protocol |
The arrays were scanned using the Affymetrix GeneChip Scanner 3000 (GSC3000) with GCOS 1.4
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Description |
gene expression data from E10.5 Lhx5 mutant mammillary neuroepithelium
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Data processing |
The data was analyzed using Expression Console 1.0 (Affymetrix) and Bioconductor package affy under R 2.7.0. Background correction, normalization and probe summarization was performed with RMA
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Submission date |
Sep 22, 2014 |
Last update date |
Aug 15, 2015 |
Contact name |
Gonzalo Alvarez-Bolado |
Organization name |
Institute for Anatomy and Cell Biology
|
Street address |
Im Neuenheimer Feld 307
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL11533 |
Series (2) |
GSE61612 |
Gene expression data from Lhx5 mutant mouse embryos |
GSE61614 |
Identification of Lhx5 binding sites and Gene expression data from Lhx5 mutant mouse embryos |
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