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Sample GSM1509394 Query DataSets for GSM1509394
Status Public on Aug 14, 2015
Title mammillary body, KO, biological rep5
Sample type RNA
 
Source name mammillary neuroepithelium of E10.5 mouse embryos
Organism Mus musculus
Characteristics tissue: brain (mammillary neuroepithelium)
strain: C57BL/6
age: E10.5
genotype: Lhx5 KO
Treatment protocol The mammillary neuroepithelium of E10.5 wild type and Lhx5 mutant embryos was dissected and immediately frozen at -80°C. The RNA was preserved with RNAlater ICE (Life Technologies).
Growth protocol Mice were crossed to obtain E10.5 wild type and Lhx5 mutant embryos.
Extracted molecule total RNA
Extraction protocol RNA was extracted by using the RNeasy Micro Kit (Qiagen) following the manufacturers instructions for animal tissue.
Label biotin
Label protocol RNA was labeled using the Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit
 
Hybridization protocol Hybridization was performed for 16 hr at 45°C on GeneChip Mouse Gene 1.1 ST arrays. GeneChips were washed and stained in the Affymetrix Fuidics Station 450.
Scan protocol The arrays were scanned using the Affymetrix GeneChip Scanner 3000 (GSC3000) with GCOS 1.4
Description gene expression data from E10.5 Lhx5 mutant mammillary neuroepithelium
Data processing The data was analyzed using Expression Console 1.0 (Affymetrix) and Bioconductor package affy under R 2.7.0. Background correction, normalization and probe summarization was performed with RMA
 
Submission date Sep 22, 2014
Last update date Aug 15, 2015
Contact name Gonzalo Alvarez-Bolado
Organization name Institute for Anatomy and Cell Biology
Street address Im Neuenheimer Feld 307
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platform ID GPL11533
Series (2)
GSE61612 Gene expression data from Lhx5 mutant mouse embryos
GSE61614 Identification of Lhx5 binding sites and Gene expression data from Lhx5 mutant mouse embryos

Data table header descriptions
ID_REF
VALUE RMA log2 transformed signal values

Data table
ID_REF VALUE
10338001 11.53580
10338002 3.41918
10338003 9.64302
10338004 9.40522
10338005 2.14645
10338006 2.33449
10338007 2.41321
10338008 2.57325
10338009 5.97582
10338010 2.15560
10338011 3.11557
10338012 2.25275
10338013 2.02557
10338014 2.12800
10338015 2.05134
10338016 4.58509
10338017 12.59970
10338018 3.75339
10338019 2.87269
10338020 5.80584

Total number of rows: 35556

Table truncated, full table size 591 Kbytes.




Supplementary file Size Download File type/resource
GSM1509394_E10R035a05.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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