NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1509393 Query DataSets for GSM1509393
Status Public on Aug 14, 2015
Title mammillary body, KO, biological rep4
Sample type RNA
 
Source name mammillary neuroepithelium of E10.5 mouse embryos
Organism Mus musculus
Characteristics tissue: brain (mammillary neuroepithelium)
strain: C57BL/6
age: E10.5
genotype: Lhx5 KO
Treatment protocol The mammillary neuroepithelium of E10.5 wild type and Lhx5 mutant embryos was dissected and immediately frozen at -80°C. The RNA was preserved with RNAlater ICE (Life Technologies).
Growth protocol Mice were crossed to obtain E10.5 wild type and Lhx5 mutant embryos.
Extracted molecule total RNA
Extraction protocol RNA was extracted by using the RNeasy Micro Kit (Qiagen) following the manufacturers instructions for animal tissue.
Label biotin
Label protocol RNA was labeled using the Ambion WT Expression kit, combined with Affymetrix GeneChip WT terminal labeling kit
 
Hybridization protocol Hybridization was performed for 16 hr at 45°C on GeneChip Mouse Gene 1.1 ST arrays. GeneChips were washed and stained in the Affymetrix Fuidics Station 450.
Scan protocol The arrays were scanned using the Affymetrix GeneChip Scanner 3000 (GSC3000) with GCOS 1.4
Description gene expression data from E10.5 Lhx5 mutant mammillary neuroepithelium
Data processing The data was analyzed using Expression Console 1.0 (Affymetrix) and Bioconductor package affy under R 2.7.0. Background correction, normalization and probe summarization was performed with RMA
 
Submission date Sep 22, 2014
Last update date Aug 15, 2015
Contact name Gonzalo Alvarez-Bolado
Organization name Institute for Anatomy and Cell Biology
Street address Im Neuenheimer Feld 307
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platform ID GPL11533
Series (2)
GSE61612 Gene expression data from Lhx5 mutant mouse embryos
GSE61614 Identification of Lhx5 binding sites and Gene expression data from Lhx5 mutant mouse embryos

Data table header descriptions
ID_REF
VALUE RMA log2 transformed signal values

Data table
ID_REF VALUE
10338001 11.21270
10338002 3.47144
10338003 9.34814
10338004 9.09144
10338005 2.15287
10338006 2.38330
10338007 2.42478
10338008 2.59245
10338009 6.19342
10338010 2.15560
10338011 3.15816
10338012 2.27826
10338013 2.05988
10338014 2.10218
10338015 2.04083
10338016 4.72316
10338017 12.44600
10338018 3.81890
10338019 2.92512
10338020 5.93303

Total number of rows: 35556

Table truncated, full table size 591 Kbytes.




Supplementary file Size Download File type/resource
GSM1509393_E10R035a04.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap