|
Status |
Public on Sep 17, 2014 |
Title |
Muscle_78 |
Sample type |
genomic |
|
|
Source name |
severely obese patient_muscle
|
Organism |
Homo sapiens |
Characteristics |
subject status: severely obese patient gender: male subject age: 52 bmi: 38.617 tissue: muscle
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from liver samples using the AllPrep DNA/RNA/Protein Mini kit (QIAgen, Hilden, Germany) according to standard instructions
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450K Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using HiScan Reader using standard recommended Illumina scanner setting
|
Description |
5822791024_R03C02
|
Data processing |
SWAN Normalization and M-value transformation, using minfi (v.1.8.9) and R (v.3.0.2)
|
|
|
Submission date |
Sep 16, 2014 |
Last update date |
Sep 17, 2014 |
Contact name |
Lude Franke |
E-mail(s) |
lude@ludesign.nl
|
Organization name |
UMCG
|
Department |
Genetics
|
Street address |
Hanzeplein 1
|
City |
Groningen |
State/province |
Please Select |
ZIP/Postal code |
9713 GZ |
Country |
Netherlands |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE61452 |
Epigenome analysis of the human muscle |
GSE61454 |
DNA methylation from severely obese samples in liver, muscle, visceral adipose tissue and subcutaneous adipose samples |
|