|
| Status |
Public on Sep 17, 2014 |
| Title |
SAT_103 |
| Sample type |
genomic |
| |
|
| Source name |
severely obese patient_Subqutaneous adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: severely obese patient
gender: male
subject age: 28
bmi: 68.67922225
tissue: Subqutaneous adipose tissue
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from liver samples using the AllPrep DNA/RNA/Protein Mini kit (QIAgen, Hilden, Germany) according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450K Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using HiScan Reader using standard recommended Illumina scanner setting
|
| Description |
SAT sample of Morbidly patient
5822791019_R06C02
|
| Data processing |
SWAN Normalization and M-value transformation, using minfi (v.1.8.9) and R (v.3.0.2)
|
| |
|
| Submission date |
Sep 16, 2014 |
| Last update date |
Sep 17, 2014 |
| Contact name |
Lude Franke |
| E-mail(s) |
lude@ludesign.nl
|
| Organization name |
UMCG
|
| Department |
Genetics
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| State/province |
Please Select |
| ZIP/Postal code |
9713 GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE61450 |
Epigenome analysis of the human subqutaneous adipose tissue |
| GSE61454 |
DNA methylation from severely obese samples in liver, muscle, visceral adipose tissue and subcutaneous adipose samples |
|
