|
| Status |
Public on Mar 05, 2007 |
| Title |
BrdU incorporation at 150min in HU in delta cds1 cells |
| Sample type |
genomic |
| |
|
| Source name |
Genomic DNA
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
Mutant strain (cdc25-22 deltacds1) was used.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Growing cdc25-22 cells expressing Thymidine Kinase were arrested at G2/M boundary and released in the presence of BrdU and HU. BrdU was incorporated in nascent DNA in following S phase. The genomic DNA was digested by HaeIII and BrdU-labeled DNA was purified in CsCl density gradient centrifugation.
|
| Label |
Biotin-11-ddATP
|
| Label protocol |
End labeling by terminal transferase
|
| |
|
| Hybridization protocol |
Standard protocol provided by Affymetrix
|
| Scan protocol |
Standard protocol provided by Affymetrix
|
| Description |
BrdU incorporation profile
|
| Data processing |
Previously described in Methods in Enzymology, Elsevier Life Sciences (CA), vol.409,chapter 23,389-410 in “DNA Repair” (edited by J.Campbell) (2006)
|
| |
|
| Submission date |
Dec 14, 2006 |
| Last update date |
Mar 04, 2007 |
| Contact name |
Katsuhiko Shirahige |
| E-mail(s) |
kshirahi@iam.u-tokyo.ac.jp
|
| Phone |
+81-3-5842-0756
|
| Fax |
+81-3-5842-0757
|
| URL |
http://www.iam.u-tokyo.ac.jp/chromosomeinformatics/
|
| Organization name |
The University of Tokyo
|
| Department |
Research Center for Epigenetic Disease
|
| Lab |
Laboratory of Genome Structure and Function
|
| Street address |
1-1-1 Yayoi
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3847 |
| Series (1) |
| GSE6523 |
Genome-wide localization of ORC and MCM binding sites and identification of replication origins in S. pombe |
|
