|
Status |
Public on Nov 03, 2014 |
Title |
genomic DNA from human adipose of subject S11a14992 |
Sample type |
genomic |
|
|
Source name |
Adipose
|
Organism |
Homo sapiens |
Characteristics |
subjectid: S11a14992 Sex: female dna.extraction.method: AllPrep age: 46 dnamage: 55.59161204 ageaccelerationresidual: 2.29891367862082 bmi: 36.9 diseasestatus: NAFLD nafldactivityscore: 2 liversteatosis: 2 liverinflammation: 0 fibrosis: 0
|
Treatment protocol |
Standard Illumina Protocol
|
Growth protocol |
DNA extracted from fresh frozen tissue
|
Extracted molecule |
genomic DNA |
Extraction protocol |
For homogenisation of five to ten milligram frozen tissue and subsequent nucleic acid isolation, tubes with 1.4 mm ceramic beads (Precellys, Villeurbanne, France) and the AllPrep DNA/RNA Mini Kit (QIAGEN, Hilden, Germany) were used.
|
Label |
Cy3, Cy5
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
genomic DNA from human adipose tissue
|
Data processing |
BeadStudio software v3.2.
|
|
|
Submission date |
Sep 09, 2014 |
Last update date |
Nov 03, 2014 |
Contact name |
Steve Horvath |
E-mail(s) |
shorvath@altoslabs.com
|
Organization name |
University of California, Los Angeles
|
Department |
Human Genetics
|
Lab |
Horvath
|
Street address |
695 Charles E. Young Drive South, Box 708822
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095-7088 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE61256 |
Obesity accelerates epigenetic aging of human liver |
GSE61257 |
Human adipose Illumina Inf 450 DNA methylation data |
|