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Sample GSM1495435 Query DataSets for GSM1495435
Status Public on Sep 30, 2014
Title EPCdEC of the second donor at passage 4 was subcultured to passage 7
Sample type RNA
 
Source name Human adult bone marrow mononuclear cell-derived endothelial progenitor outgrowth cells (EPOCs): lot B112057, BioChain Institute, Inc., Hayward, CA
Organism Homo sapiens
Characteristics cell type: vascular endothelical cells
Growth protocol Vascular endothelia cells were cultured on 0.1% gelatin-coated plates using EGM®-2 BulletKit (Lonza Group Ltd. Basel, Switzerland) . Cells were subcultured at split ratios of 1:2 ~ 1:3 twice a week.
Extracted molecule total RNA
Extraction protocol Total RNAs were isolated by using TRIzoL® Reagent (Life Technologies, Inc., Grand Island, NY).
Label biotin
Label protocol Samples were labeled according to the Affymetrix standard protocol.
 
Hybridization protocol Samples were hybridized according to the Affymetrix standard protocol.
Scan protocol An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
Data processing Normalized expression signal data was generated at the exon level using Affymetrix's Expression Console by RMA.
 
Submission date Sep 02, 2014
Last update date Sep 30, 2014
Contact name Kumiko Saeki
Organization name National Center for Global Health and Medicine
Department Department of disease control
Street address 1-21-1 Toyama
City Shinjuku-ku
State/province Tokyo
ZIP/Postal code 162-8655
Country Japan
 
Platform ID GPL10739
Series (1)
GSE61000 Effects of differences in donors and passage numbers of endothelial progenitor cell (EPC)-derived vascular endothelial cells (VECs) on the phenotype determination of VECs (type-I versus type-II).
Relations
Alternative to GSM1495432 (gene-level analysis)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
7892501 69.90554541
7892502 19.64682117
7892503 8.560909056
7892504 418.6227771
7892505 14.94230696
7892506 14.56343731
7892507 25.30223637
7892508 16.64178177
7892509 5158.514656
7892510 14.75265795
7892511 9.850029191
7892512 93.3668173
7892513 18.94736077
7892514 1953.627909
7892515 437.9532302
7892516 59.87583645
7892517 18.17737538
7892518 5.309816484
7892519 43.68039508
7892520 500.4142283

Total number of rows: 257430

Table truncated, full table size 4995 Kbytes.




Supplementary file Size Download File type/resource
GSM1495435_PF091217_02.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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