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Sample GSM1494890 Query DataSets for GSM1494890
Status Public on Nov 06, 2015
Title ampullaP_4 [miRNA]
Sample type RNA
 
Source name Fresh frozen tumor
Organism Homo sapiens
Characteristics tissue: ampulla pancreatobiliary type
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
Label Cy3
Label protocol miRNA was labeled with Cy-3 according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies, using 200 ng total RNA as input.
 
Hybridization protocol miRNA microarrays Cy-3-labeled cRNA was hybridized according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies.
Scan protocol The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
Description Gene expression of fresh frozen ampulla pancreatobiliary type patient 4
Data processing The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles was RMA normalized for miRNA expression data. Filtering of controls, unannotated genes and low expressed probes was done.
 
Submission date Sep 02, 2014
Last update date Nov 06, 2015
Contact name Vandana Sandhu
E-mail(s) vansan@rr-research.no
Organization name Radiumhospitalet, Oslo
Department Department of Genetics
Street address Ullernchausseen 70
City Oslo
State/province oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL15159
Series (2)
GSE60978 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA]
GSE60980 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_25_P00015345 4.602303208
A_25_P00015343 5.073462668
Blank 4.327580445
dmr_285 11.70344704
dmr_3 14.47864273
dmr_308 4.629534913
dmr_316 4.725075113
dmr_31a 10.99306297
dmr_6 14.05964446
A_25_P00013722 4.552782397
A_25_P00013718 4.779685032
A_25_P00011668 4.753107552
A_25_P00013809 4.579003941
A_25_P00011834 4.727509311
A_25_P00013749 4.63582623
A_25_P00013752 5.067966893
A_25_P00011853 7.256314806
A_25_P00013814 4.519056974
A_25_P00014783 4.677958136
A_25_P00011886 4.565534651

Total number of rows: 1368

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM1494890_miRNA_ampullaP_4.txt.gz 8.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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