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Sample GSM1494868 Query DataSets for GSM1494868
Status Public on Nov 06, 2015
Title PDAC_50 [miRNA]
Sample type RNA
 
Source name Fresh frozen tumor
Organism Homo sapiens
Characteristics tissue: pancreatic ductal adenocarcinoma
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
Label Cy3
Label protocol miRNA was labeled with Cy-3 according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies, using 200 ng total RNA as input.
 
Hybridization protocol miRNA microarrays Cy-3-labeled cRNA was hybridized according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies.
Scan protocol The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
Description Gene expression of fresh frozen pancreatic ductal adenocarcinoma patient 50
Data processing The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles was RMA normalized for miRNA expression data. Filtering of controls, unannotated genes and low expressed probes was done.
 
Submission date Sep 02, 2014
Last update date Nov 06, 2015
Contact name Vandana Sandhu
E-mail(s) vansan@rr-research.no
Organization name Radiumhospitalet, Oslo
Department Department of Genetics
Street address Ullernchausseen 70
City Oslo
State/province oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL15159
Series (2)
GSE60978 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA]
GSE60980 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_25_P00015345 4.611915813
A_25_P00015343 4.837107125
Blank 4.485260896
dmr_285 11.70965385
dmr_3 13.69182401
dmr_308 4.702970334
dmr_316 4.799371201
dmr_31a 11.18110792
dmr_6 12.68683689
A_25_P00013722 4.653280733
A_25_P00013718 4.665264578
A_25_P00011668 4.616749941
A_25_P00013809 4.684866762
A_25_P00011834 4.59844806
A_25_P00013749 4.695183597
A_25_P00013752 4.828887128
A_25_P00011853 5.755830647
A_25_P00013814 4.628443193
A_25_P00014783 4.619774614
A_25_P00011886 4.657722108

Total number of rows: 1368

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM1494868_miRNA_PDAC_50.txt.gz 8.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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