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Sample GSM1494867 Query DataSets for GSM1494867
Status Public on Nov 06, 2015
Title PDAC_49 [miRNA]
Sample type RNA
 
Source name Fresh frozen tumor
Organism Homo sapiens
Characteristics tissue: pancreatic ductal adenocarcinoma
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
Label Cy3
Label protocol miRNA was labeled with Cy-3 according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies, using 200 ng total RNA as input.
 
Hybridization protocol miRNA microarrays Cy-3-labeled cRNA was hybridized according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies.
Scan protocol The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
Description Gene expression of fresh frozen pancreatic ductal adenocarcinoma patient 49
Data processing The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles was RMA normalized for miRNA expression data. Filtering of controls, unannotated genes and low expressed probes was done.
 
Submission date Sep 02, 2014
Last update date Nov 06, 2015
Contact name Vandana Sandhu
E-mail(s) vansan@rr-research.no
Organization name Radiumhospitalet, Oslo
Department Department of Genetics
Street address Ullernchausseen 70
City Oslo
State/province oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL15159
Series (2)
GSE60978 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA]
GSE60980 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_25_P00015345 4.651190023
A_25_P00015343 4.695402138
Blank 4.259615465
dmr_285 11.96804869
dmr_3 14.33053356
dmr_308 4.709783449
dmr_316 4.737853229
dmr_31a 11.29835015
dmr_6 13.12081944
A_25_P00013722 4.631112335
A_25_P00013718 4.677965899
A_25_P00011668 4.641925377
A_25_P00013809 4.735538523
A_25_P00011834 4.614304372
A_25_P00013749 4.68492452
A_25_P00013752 4.878466127
A_25_P00011853 5.647542067
A_25_P00013814 4.617828376
A_25_P00014783 4.65794322
A_25_P00011886 4.636755938

Total number of rows: 1368

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM1494867_miRNA_PDAC_49.txt.gz 8.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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