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Sample GSM1494864 Query DataSets for GSM1494864
Status Public on Nov 06, 2015
Title PDAC_46 [miRNA]
Sample type RNA
 
Source name Fresh frozen tumor
Organism Homo sapiens
Characteristics tissue: pancreatic ductal adenocarcinoma
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
Label Cy3
Label protocol miRNA was labeled with Cy-3 according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies, using 200 ng total RNA as input.
 
Hybridization protocol miRNA microarrays Cy-3-labeled cRNA was hybridized according to the manual miRNA Microarray System with miRNA Complete Labeling and Hyb Kit (version 2.4, September 2011) from Agilent Technologies.
Scan protocol The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
Description Gene expression of fresh frozen pancreatic ductal adenocarcinoma patient 46
Data processing The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles was RMA normalized for miRNA expression data. Filtering of controls, unannotated genes and low expressed probes was done.
 
Submission date Sep 02, 2014
Last update date Nov 06, 2015
Contact name Vandana Sandhu
E-mail(s) vansan@rr-research.no
Organization name Radiumhospitalet, Oslo
Department Department of Genetics
Street address Ullernchausseen 70
City Oslo
State/province oslo
ZIP/Postal code 0310
Country Norway
 
Platform ID GPL15159
Series (2)
GSE60978 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [miRNA]
GSE60980 mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_25_P00015345 4.623386329
A_25_P00015343 4.752039283
Blank 4.238257098
dmr_285 11.98252393
dmr_3 14.18638042
dmr_308 4.76171403
dmr_316 4.826262432
dmr_31a 11.76383158
dmr_6 13.63220346
A_25_P00013722 4.684880573
A_25_P00013718 4.654698625
A_25_P00011668 4.67314014
A_25_P00013809 4.783225991
A_25_P00011834 4.572430381
A_25_P00013749 4.70554196
A_25_P00013752 5.069464064
A_25_P00011853 6.237508023
A_25_P00013814 4.588941273
A_25_P00014783 4.660814993
A_25_P00011886 4.588534765

Total number of rows: 1368

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM1494864_miRNA_PDAC_46.txt.gz 8.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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