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Sample GSM1487580 Query DataSets for GSM1487580
Status Public on Aug 27, 2014
Title C4 vs. S4
Sample type RNA
 
Channel 1
Source name C4
Organism Homo sapiens
Characteristics cell type: primary cancer cell of ovarian serous carcinoma
Growth protocol Serum-free DMEM/F12 medium supplemented with 20 ng/ml epidermal growth factor,10 ng/ml basic fibroblast growth factor, 0.4% bovine serum albumin, and 5 µg/mL insulin.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol 0.2ug of total RNA was labeled using Low Input Quick Amp Labeling Kit, Two-Color (Agilent Technology) as following manufacturer's instructions.
 
Channel 2
Source name S4
Organism Homo sapiens
Characteristics cell type: Isolated spheroid-forming cell of ovarian serous carcinoma
Growth protocol Serum-free DMEM/F12 medium supplemented with 20 ng/ml epidermal growth factor,10 ng/ml basic fibroblast growth factor, 0.4% bovine serum albumin, and 5 µg/mL insulin.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol 0.2ug of total RNA was labeled using Low Input Quick Amp Labeling Kit, Two-Color (Agilent Technology) as following manufacturer's instructions.
 
 
Hybridization protocol Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
Scan protocol Scanned on an Agilent G2565AA scanner.
Description Ovarian serous carcinoma_no4_Spheroid-forming cell vs. Corresponding primary cancer cell
Data processing Agilent Feature Extraction Software (v 9.3.2.1) was used for background subtraction and LOWESS normalization.
 
Submission date Aug 26, 2014
Last update date Apr 23, 2018
Contact name Ah-Young Kwon
Organization name College of Medicine, CHA University
Department Department of Pathology
Street address 351 Yatap-dong, Bundang-gu
City Seongnam-si
State/province Gyeonggi-do
ZIP/Postal code 463-712
Country South Korea
 
Platform ID GPL17077
Series (1)
GSE60765 Identification of genes differentailly expressed in cancer stem-like cells of ovarian serous carcinoma and its clinical significance
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE log2 of RATIO
RATIO The averages of normalized ratios were calculated by dividing the average of normalized signal channel intensity by the average of normalized control channel intensity.

Data table
ID_REF VALUE RATIO
A_19_P00315452 -3.2536 0.10485158
A_19_P00315459 -0.2009 0.86998695
A_19_P00315482 -0.4087 0.75331783
A_19_P00315492 -1.5528 0.34084213
A_19_P00315493 0.1188 1.0857997
A_19_P00315502 -1.2222 0.42861882
A_19_P00315506 -0.7995 0.5745635
A_19_P00315518 -1.0306 0.48949024
A_19_P00315519 -0.2271 0.854348
A_19_P00315524 6.2309 75.10984
A_19_P00315528 -0.5495 0.6832563
A_19_P00315529 -1.1923 0.43761176
A_19_P00315538 0.7408 1.6711344
A_19_P00315541 -0.3470 0.78622675
A_19_P00315543 1.4574 2.7460654
A_19_P00315550 -0.6889 0.6203421
A_19_P00315551 -0.5672 0.6749331
A_19_P00315554 0.3667 1.2893634
A_19_P00315581 0.7063 1.6316599
A_19_P00315583 -0.1864 0.8788076

Total number of rows: 50599

Table truncated, full table size 1546 Kbytes.




Supplementary file Size Download File type/resource
GSM1487580_C4_vs._S4.txt.gz 6.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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