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Sample GSM1484435 Query DataSets for GSM1484435
Status Public on Jan 04, 2017
Title CGVHD_25
Sample type RNA
 
Source name CGVHD
Organism Homo sapiens
Characteristics cell type: Monocyte
disease status: CGVHD
Growth protocol Monocytes from cryopreserved PBMC of normal and CGVHD patients were enriched by gating on FSChi SSChi CD3– CD4dim cells using an Influx Cell Sorter (Becton Dickinson Immunocytometry Systems)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from monocytes by using RNeasy kit (Qiagen, Germantown, , MD, USA)
Label Biotin
Label protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
 
Hybridization protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
Scan protocol scanning was carried out with the GeneChip Scanner 3000 and image analysis with the Affymetrix GeneChip Command Console Scan Control(all from Affymetrix, Santa Clara, CA).
Description gene expression data
Data processing Expression data were normalized, background-corrected, and summarized using the RNA algorithm
 
Submission date Aug 22, 2014
Last update date Jan 04, 2017
Contact name PING JIN
E-mail(s) PJIN@CC.NIH.GOV
Organization name CC/DTM/NIH
Department DTM
Lab CCE
Street address 9000 ROCKVILLE PIKE
City BETHESDA
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10739
Series (1)
GSE60674 Upregulation of Interferon-inducible and damage response pathways in chronic graft-versus-host disease

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
7892501 5.67067
7892502 7.07662
7892503 4.89221
7892504 9.70479
7892505 4.23516
7892506 7.44573
7892507 5.34614
7892508 8.6558
7892509 10.1718
7892510 5.33521
7892511 4.40157
7892512 7.46139
7892513 4.45204
7892514 11.1732
7892515 10.7752
7892516 3.76925
7892517 7.44717
7892518 3.28901
7892519 6.57958
7892520 10.669

Total number of rows: 257430

Table truncated, full table size 3991 Kbytes.




Supplementary file Size Download File type/resource
GSM1484435_Ping35_HuGene-1_0-st-v1_.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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