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Sample GSM1484425 Query DataSets for GSM1484425
Status Public on Jan 04, 2017
Title CGVHD_15
Sample type RNA
 
Source name CGVHD
Organism Homo sapiens
Characteristics cell type: Monocyte
disease status: CGVHD
Growth protocol Monocytes from cryopreserved PBMC of normal and CGVHD patients were enriched by gating on FSChi SSChi CD3– CD4dim cells using an Influx Cell Sorter (Becton Dickinson Immunocytometry Systems)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from monocytes by using RNeasy kit (Qiagen, Germantown, , MD, USA)
Label Biotin
Label protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
 
Hybridization protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
Scan protocol scanning was carried out with the GeneChip Scanner 3000 and image analysis with the Affymetrix GeneChip Command Console Scan Control(all from Affymetrix, Santa Clara, CA).
Description gene expression data
Data processing Expression data were normalized, background-corrected, and summarized using the RNA algorithm
 
Submission date Aug 22, 2014
Last update date Jan 04, 2017
Contact name PING JIN
E-mail(s) PJIN@CC.NIH.GOV
Organization name CC/DTM/NIH
Department DTM
Lab CCE
Street address 9000 ROCKVILLE PIKE
City BETHESDA
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10739
Series (1)
GSE60674 Upregulation of Interferon-inducible and damage response pathways in chronic graft-versus-host disease

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
7892501 5.59436
7892502 7.4515
7892503 3.89793
7892504 9.90107
7892505 6.04756
7892506 6.96014
7892507 5.61768
7892508 8.41568
7892509 10.5938
7892510 5.61917
7892511 5.15549
7892512 7.35839
7892513 4.96263
7892514 10.8609
7892515 10.6275
7892516 4.40623
7892517 7.96744
7892518 4.11751
7892519 6.51123
7892520 10.3292

Total number of rows: 257430

Table truncated, full table size 3995 Kbytes.




Supplementary file Size Download File type/resource
GSM1484425_Ping25_HuGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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