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Sample GSM1484415 Query DataSets for GSM1484415
Status Public on Jan 04, 2017
Title CGVHD_5
Sample type RNA
 
Source name CGVHD
Organism Homo sapiens
Characteristics cell type: Monocyte
disease status: CGVHD
Growth protocol Monocytes from cryopreserved PBMC of normal and CGVHD patients were enriched by gating on FSChi SSChi CD3– CD4dim cells using an Influx Cell Sorter (Becton Dickinson Immunocytometry Systems)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from monocytes by using RNeasy kit (Qiagen, Germantown, , MD, USA)
Label Biotin
Label protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
 
Hybridization protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
Scan protocol scanning was carried out with the GeneChip Scanner 3000 and image analysis with the Affymetrix GeneChip Command Console Scan Control(all from Affymetrix, Santa Clara, CA).
Description gene expression data
Data processing Expression data were normalized, background-corrected, and summarized using the RNA algorithm
 
Submission date Aug 22, 2014
Last update date Jan 04, 2017
Contact name PING JIN
E-mail(s) PJIN@CC.NIH.GOV
Organization name CC/DTM/NIH
Department DTM
Lab CCE
Street address 9000 ROCKVILLE PIKE
City BETHESDA
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10739
Series (1)
GSE60674 Upregulation of Interferon-inducible and damage response pathways in chronic graft-versus-host disease

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
7892501 4.73996
7892502 6.53088
7892503 5.85064
7892504 9.75887
7892505 4.07358
7892506 7.08775
7892507 5.43155
7892508 9.01582
7892509 10.0649
7892510 5.08171
7892511 4.82222
7892512 7.55456
7892513 5.09879
7892514 10.8817
7892515 10.6659
7892516 3.80496
7892517 8.72726
7892518 3.51379
7892519 6.80608
7892520 10.4204

Total number of rows: 257430

Table truncated, full table size 3996 Kbytes.




Supplementary file Size Download File type/resource
GSM1484415_Ping15_HuGene-1_0-st-v1_.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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