NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1484405 Query DataSets for GSM1484405
Status Public on Jan 04, 2017
Title ND_3
Sample type RNA
 
Source name ND
Organism Homo sapiens
Characteristics cell type: Monocyte
disease status: healthy
Growth protocol Monocytes from cryopreserved PBMC of normal and CGVHD patients were enriched by gating on FSChi SSChi CD3– CD4dim cells using an Influx Cell Sorter (Becton Dickinson Immunocytometry Systems)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from monocytes by using RNeasy kit (Qiagen, Germantown, , MD, USA)
Label Biotin
Label protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
 
Hybridization protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
Scan protocol scanning was carried out with the GeneChip Scanner 3000 and image analysis with the Affymetrix GeneChip Command Console Scan Control(all from Affymetrix, Santa Clara, CA).
Description gene expression data
Data processing Expression data were normalized, background-corrected, and summarized using the RNA algorithm
 
Submission date Aug 22, 2014
Last update date Jan 04, 2017
Contact name PING JIN
E-mail(s) PJIN@CC.NIH.GOV
Organization name CC/DTM/NIH
Department DTM
Lab CCE
Street address 9000 ROCKVILLE PIKE
City BETHESDA
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10739
Series (1)
GSE60674 Upregulation of Interferon-inducible and damage response pathways in chronic graft-versus-host disease

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
7892501 5.26928
7892502 6.62681
7892503 3.96621
7892504 9.34712
7892505 4.13062
7892506 7.66861
7892507 5.69493
7892508 8.03657
7892509 10.2584
7892510 4.3878
7892511 4.45867
7892512 7.83201
7892513 3.75279
7892514 11.2709
7892515 9.73162
7892516 3.07415
7892517 7.07714
7892518 3.12564
7892519 6.43868
7892520 9.99031

Total number of rows: 257430

Table truncated, full table size 3994 Kbytes.




Supplementary file Size Download File type/resource
GSM1484405_Ping-05_HuGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap