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Sample GSM1484403 Query DataSets for GSM1484403
Status Public on Jan 04, 2017
Title ND_1
Sample type RNA
 
Source name ND
Organism Homo sapiens
Characteristics cell type: Monocyte
disease status: healthy
Growth protocol Monocytes from cryopreserved PBMC of normal and CGVHD patients were enriched by gating on FSChi SSChi CD3– CD4dim cells using an Influx Cell Sorter (Becton Dickinson Immunocytometry Systems)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from monocytes by using RNeasy kit (Qiagen, Germantown, , MD, USA)
Label Biotin
Label protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
 
Hybridization protocol 300ng of total RNA per sample was amplified according to manufacturer's instructions (Ambion WT Expression Kit). Next cDNAs were fragmented, biotinylated, and hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix WT Terminal Labeling Kit).
Scan protocol scanning was carried out with the GeneChip Scanner 3000 and image analysis with the Affymetrix GeneChip Command Console Scan Control(all from Affymetrix, Santa Clara, CA).
Description gene expression data
Data processing Expression data were normalized, background-corrected, and summarized using the RNA algorithm
 
Submission date Aug 22, 2014
Last update date Jan 04, 2017
Contact name PING JIN
E-mail(s) PJIN@CC.NIH.GOV
Organization name CC/DTM/NIH
Department DTM
Lab CCE
Street address 9000 ROCKVILLE PIKE
City BETHESDA
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL10739
Series (1)
GSE60674 Upregulation of Interferon-inducible and damage response pathways in chronic graft-versus-host disease

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
7892501 3.79212
7892502 6.4282
7892503 5.17541
7892504 9.45252
7892505 5.2065
7892506 7.35204
7892507 5.24261
7892508 8.42169
7892509 10.1201
7892510 5.39271
7892511 4.69281
7892512 7.63226
7892513 3.61061
7892514 10.8721
7892515 10.2681
7892516 3.63897
7892517 6.8311
7892518 3.0367
7892519 6.54436
7892520 10.4304

Total number of rows: 257430

Table truncated, full table size 3991 Kbytes.




Supplementary file Size Download File type/resource
GSM1484403_Ping-03_HuGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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