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Sample GSM1483081 Query DataSets for GSM1483081
Status Public on Sep 01, 2014
Title Whole blood_001-130-002-2007-B
Sample type genomic
Source name whole blood (dried blood spots)
Organism Homo sapiens
Characteristics gender: Female
race: African_american
normalizedpbconc: 10.47605773
age (in months): 48
mother's age (in months): 264
gestational age (in months): 216
smoking (0=no, 1=yes): 0
Treatment protocol NA
Extracted molecule genomic DNA
Extraction protocol DNA was isolated from dried blood spots with Qiagen EZ1 Advanced® using the DNA Investigator® reagents and protocol card. The “Stains on Fabric” preprocessing and Trace® (tip-dance) instrument protocol was used for isolation.
Label Cy3, Cy5
Label protocol As described by Illumina for Human methylation 450K assay
Hybridization protocol As described by Illumina for Human methylation 450K assay
Scan protocol As described by Illumina for Human methylation 450K assay
Description Dried blood spots for Pb and DNA methylation study
Data processing Sample normalization done using Bioconductor package ChAMP. Type1 to type 2 probe bias correction was done using Beta mixed interquantile range normalization. Based on gender differences in DNA methylation profile the representative DNA methylation loci was divided into conserved female specific and male specific regions. The CpG sites show highly correlated changes in methylation signatures and can be defined as co-regulated regions. The co-regulated regions were assigned to specified clusters using the A-clustering algorithm (Sofer et al 2013). The association between Pb concentration measured in dried blood spot and DNA methylation of coregulated region for conserved female specific and male specific regions was calculated using Generalized estimating equations (GEE) (Sofer et al 2013)
Rawdata_signal_intensities.txt reports Unmethylated and methylated signal intensities (Pre-normalization)
Submission date Aug 21, 2014
Last update date Sep 01, 2014
Contact name Douglas Ruden
Phone 3135776688
Organization name Wayne State University
Department Department of Obstetrics and Gynecology
Street address 275 E. Hancock Ave., Room 291.1
City Detroit
State/province Michigan
ZIP/Postal code 48201
Country USA
Platform ID GPL13534
Series (1)
GSE60598 Early life Lead exposure causes distinct gender specific changes in the DNA methylation profile of DNA extracted from dried blood spots

Data table header descriptions
VALUE normalized Average Beta

Data table
cg00001349 0.832909609091739
cg00001583 0.0365787568937292
cg00002028 0.0308431203097047
cg00002719 0.0311918754764622
cg00002837 0.377302507261366
cg00003202 0.0111109827723978
cg00003287 0.191005866047592
cg00007036 0.959152203028494
cg00007898 0.0187533109749666
cg00008004 0.0534695003335743
cg00008647 0.0937340255997367
cg00009292 0.0999735354153524
cg00011717 0.944411445942857
cg00014168 0.701798071027746
cg00015286 0.866694534252799
cg00015930 0.972156512365049
cg00016238 0.94009289898094
cg00017221 0.0371366864693764
cg00017437 0.0477418380792214
cg00021576 0.0520807875399837

Total number of rows: 378548

Table truncated, full table size 10817 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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