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Sample GSM1483073 Query DataSets for GSM1483073
Status Public on Sep 01, 2014
Title Whole blood_001-092-001-2009-B
Sample type genomic
Source name whole blood (dried blood spots)
Organism Homo sapiens
Characteristics gender: Male
race: African_american
normalizedpbconc: 8.840076423
age (in months): 36
mother's age (in months): 252
gestational age (in months): 216
smoking (0=no, 1=yes): 0
Treatment protocol NA
Extracted molecule genomic DNA
Extraction protocol DNA was isolated from dried blood spots with Qiagen EZ1 Advanced® using the DNA Investigator® reagents and protocol card. The “Stains on Fabric” preprocessing and Trace® (tip-dance) instrument protocol was used for isolation.
Label Cy3, Cy5
Label protocol As described by Illumina for Human methylation 450K assay
Hybridization protocol As described by Illumina for Human methylation 450K assay
Scan protocol As described by Illumina for Human methylation 450K assay
Description Dried blood spots for Pb and DNA methylation study
Data processing Sample normalization done using Bioconductor package ChAMP. Type1 to type 2 probe bias correction was done using Beta mixed interquantile range normalization. Based on gender differences in DNA methylation profile the representative DNA methylation loci was divided into conserved female specific and male specific regions. The CpG sites show highly correlated changes in methylation signatures and can be defined as co-regulated regions. The co-regulated regions were assigned to specified clusters using the A-clustering algorithm (Sofer et al 2013). The association between Pb concentration measured in dried blood spot and DNA methylation of coregulated region for conserved female specific and male specific regions was calculated using Generalized estimating equations (GEE) (Sofer et al 2013)
Rawdata_signal_intensities.txt reports Unmethylated and methylated signal intensities (Pre-normalization)
Submission date Aug 21, 2014
Last update date Sep 01, 2014
Contact name Douglas Ruden
Phone 3135776688
Organization name Wayne State University
Department Department of Obstetrics and Gynecology
Street address 275 E. Hancock Ave., Room 291.1
City Detroit
State/province Michigan
ZIP/Postal code 48201
Country USA
Platform ID GPL13534
Series (1)
GSE60598 Early life Lead exposure causes distinct gender specific changes in the DNA methylation profile of DNA extracted from dried blood spots

Data table header descriptions
VALUE normalized Average Beta

Data table
cg00001349 0.908920386911521
cg00001583 0.0466279607934313
cg00002028 0.0226515347657171
cg00002719 0.0331282784730395
cg00002837 0.374044955322135
cg00003202 0.0114167575290985
cg00003287 0.15735801728252
cg00007036 0.963437412472561
cg00007898 0.0248927719709525
cg00008004 0.0515089819445921
cg00008647 0.111944364402861
cg00009292 0.112577769331632
cg00011717 0.959509545681194
cg00014168 0.769764323372323
cg00015286 0.873127936296138
cg00015930 0.977140787376722
cg00016238 0.901566296796839
cg00017221 0.0318286517027276
cg00017437 0.0515259258792837
cg00021576 0.0388724158204178

Total number of rows: 378548

Table truncated, full table size 10815 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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