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Sample GSM1483048 Query DataSets for GSM1483048
Status Public on Sep 01, 2014
Title Whole blood_001-113-002-2012-B
Sample type genomic
Source name whole blood (dried blood spots)
Organism Homo sapiens
Characteristics gender: Female
race: African_american
normalizedpbconc: 4.602492159
age (in months): 8.000000004
mother's age (in months): 228
gestational age (in months): 208
smoking (0=no, 1=yes): 0
Treatment protocol NA
Extracted molecule genomic DNA
Extraction protocol DNA was isolated from dried blood spots with Qiagen EZ1 Advanced® using the DNA Investigator® reagents and protocol card. The “Stains on Fabric” preprocessing and Trace® (tip-dance) instrument protocol was used for isolation.
Label Cy3, Cy5
Label protocol As described by Illumina for Human methylation 450K assay
Hybridization protocol As described by Illumina for Human methylation 450K assay
Scan protocol As described by Illumina for Human methylation 450K assay
Description Dried blood spots for Pb and DNA methylation study
Data processing Sample normalization done using Bioconductor package ChAMP. Type1 to type 2 probe bias correction was done using Beta mixed interquantile range normalization. Based on gender differences in DNA methylation profile the representative DNA methylation loci was divided into conserved female specific and male specific regions. The CpG sites show highly correlated changes in methylation signatures and can be defined as co-regulated regions. The co-regulated regions were assigned to specified clusters using the A-clustering algorithm (Sofer et al 2013). The association between Pb concentration measured in dried blood spot and DNA methylation of coregulated region for conserved female specific and male specific regions was calculated using Generalized estimating equations (GEE) (Sofer et al 2013)
Rawdata_signal_intensities.txt reports Unmethylated and methylated signal intensities (Pre-normalization)
Submission date Aug 21, 2014
Last update date Sep 01, 2014
Contact name Douglas Ruden
Phone 3135776688
Organization name Wayne State University
Department Department of Obstetrics and Gynecology
Street address 275 E. Hancock Ave., Room 291.1
City Detroit
State/province Michigan
ZIP/Postal code 48201
Country USA
Platform ID GPL13534
Series (1)
GSE60598 Early life Lead exposure causes distinct gender specific changes in the DNA methylation profile of DNA extracted from dried blood spots

Data table header descriptions
VALUE normalized Average Beta

Data table
cg00001349 0.794487413826843
cg00001583 0.0200345946674859
cg00002028 0.0319568733212715
cg00002719 0.0514448942299266
cg00002837 0.38627147083384
cg00003202 0.00804135498464801
cg00003287 0.155319244648001
cg00007036 0.958254990386166
cg00007898 0.0186015805530738
cg00008004 0.0697513170908361
cg00008647 0.0588552800807858
cg00009292 0.0870736820733719
cg00011717 0.936329759026485
cg00014168 0.675607797945134
cg00015286 0.774771978821877
cg00015930 0.973491396148632
cg00016238 0.8385139838811
cg00017221 0.0428518997569278
cg00017437 0.0381965517458208
cg00021576 0.0298021159033962

Total number of rows: 378548

Table truncated, full table size 10818 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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