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Sample GSM1481336 Query DataSets for GSM1481336
Status Public on Aug 20, 2014
Title #1 WT
Sample type RNA
 
Source name WT
Organism Mus musculus
Characteristics backgroud strain: 129 x FVBn x C57BL/6
cell type: pancreatic islets
Treatment protocol Mice were maintained in a pathogen-free facility, with 4–5 animals per cage in a temperature-controlled room with a 12-h light/dark cycle and access to food and water ad libitum. Age and sex matched littermate cohorts were used for all the experiments to account for potential strain differences.
Growth protocol All animal studies were approved by the Columbia Animal Care and Use Committee.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Labeled cRNA were prepared according to the standard Nugen protocol.
 
Hybridization protocol Hybridization was performed by Columbia University microarray core facility. Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scanning was performed by Columbia University microarray core facility. GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing RMA by Expression Console
 
Submission date Aug 19, 2014
Last update date Aug 20, 2014
Contact name Domenico Accili
E-mail(s) da230@columbia.edu
Organization name Columbia University
Street address 1150 St. Nicholas Ave
City NEW YORK
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL6096
Series (1)
GSE60505 Metabolic Inflexibility Impairs Insulin Secretion And Results In MODY-like Diabetes In Triple FoxO-deficient Mice

Data table header descriptions
ID_REF
VALUE log2-rma-gene-core

Data table
ID_REF VALUE
6848511 9.18153
6864895 10.3027
6766590 4.2775
6914045 4.13333
6963197 5.88357
6766588 4.26753
6995964 8.72024
6766587 3.58056
6815739 9.11013
6766586 8.07844
7012346 4.92883
6766585 6.66995
6848505 7.34211
6766584 5.42728
6848504 8.60167
6979576 5.25123
6995960 6.31291
6766583 4.8474
6963191 7.20287
6979575 6.80947

Total number of rows: 23238

Table truncated, full table size 360 Kbytes.




Supplementary file Size Download File type/resource
GSM1481336__1_WT.CEL.gz 24.6 Mb (ftp)(http) CEL
GSM1481336__1_WT.rma-gene-core.chp.gz 164.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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